Development of Chitosan Particles Loaded with siRNA for Cystatin C to Control Intracellular Drug-Resistant Mycobacterium tuberculosis.

The golden age of antibiotics for tuberculosis (TB) is marked by its success in the 1950s of the last century. However, TB is not under control, and the rise in antibiotic resistance worldwide is a major threat to global health care. Understanding the complex interactions between TB bacilli and their host can inform the rational design of better TB therapeutics, including vaccines, new antibiotics, and host-directed therapies. We recently demonstrated that the modulation of cystatin C in human macrophages via RNA silencing improved the anti-mycobacterial immune responses to Mycobacterium tuberculosis infection. Available in vitro transfection methods are not suitable for the clinical translation of host-cell RNA silencing. To overcome this limitation, we developed different RNA delivery systems (DSs) that target human macrophages. Human peripheral blood-derived macrophages and THP1 cells are difficult to transfect using available methods. In this work, a new potential nanomedicine based on chitosan (CS-DS) was efficiently developed to carry a siRNA-targeting cystatin C to the infected macrophage models. Consequently, an effective impact on the intracellular survival/replication of TB bacilli, including drug-resistant clinical strains, was observed. Altogether, these results suggest the potential use of CS-DS in adjunctive therapy for TB in combination or not with antibiotics.

Sailing Uncharted Waters with Old Boats? COVID-19 and the Digitalization and Professionalization of Presidential Campaigns in Portugal

This article investigates the extent to which the COVID-19 pandemic fostered significant shifts in election campaigning. The argument is that COVID-19 might have had an impact on both digitalization and professionalization, which might have been regarded as necessary strategies to curb the difficulties brought about by the pandemic. We apply a most similar systems design with a threefold comparative scheme in order to capture and isolate such effects in the campaigns preceding the 2021 Portuguese presidential elections, using data from campaign spending, campaign activities, and social media activity and impact. Results show that the pandemic crisis has not, generally speaking, brought about a higher level of digitalization of electoral campaigns, in spite of online events having become more common. On the contrary, while there were signs of feebler patterns of normalization of online competition in 2021 vis-à-vis 2016, namely in terms of engagement, normalization was stronger after the lockdown than before. Lastly, relative investment in professionalization was similar in 2016 and 2021, and the difference between the budgeted and the actual investment in 2021 cannot be attributed to the worsening of the pandemic situation or to the lockdown. In sum, we depict a scenario of remarkable stability of the electoral campaigns put forward by presidential candidates in terms of digitalization and professionalization. Its possible causes and consequences are discussed.

pyKVFinder: an efficient and integrable Python package for biomolecular cavity detection and characterization in data science.

BACKGROUND: Biomolecular interactions that modulate biological processes occur mainly in cavities throughout the surface of biomolecular structures. In the data science era, structural biology has benefited from the increasing availability of biostructural data due to advances in structural determination and computational methods. In this scenario, data-intensive cavity analysis demands efficient scripting routines built on easily manipulated data structures. To fulfill this need, we developed pyKVFinder, a Python package to detect and characterize cavities in biomolecular structures for data science and automated pipelines. RESULTS: pyKVFinder efficiently detects cavities in biomolecular structures and computes their volume, area, depth and hydropathy, storing these cavity properties in NumPy arrays. Benefited from Python ecosystem interoperability and data structures, pyKVFinder can be integrated with third-party scientific packages and libraries for mathematical calculations, machine learning and 3D visualization in automated workflows. As proof of pyKVFinder's capabilities, we successfully identified and compared ADRP substrate-binding site of SARS-CoV-2 and a set of homologous proteins with pyKVFinder, showing its integrability with data science packages such as matplotlib, NGL Viewer, SciPy and Jupyter notebook. CONCLUSIONS: We introduce an efficient, highly versatile and easily integrable software for detecting and characterizing biomolecular cavities in data science applications and automated protocols. pyKVFinder facilitates biostructural data analysis with scripting routines in the Python ecosystem and can be building blocks for data science and drug design applications.

The Influence of Noise in the Neurofeedback Training Sessions in Student Athletes.

Considering that athletes constantly practice and compete in noisy environments, the aim was to investigate if performing neurofeedback training in these conditions would yield better results in performance than in silent ones. A total of forty-five student athletes aged from 18 to 35 years old and divided equally into three groups participated in the experiment (mean ± SD for age: 22.02 ± 3.05 years). The total neurofeedback session time for each subject was 300 min and were performed twice a week. The environment in which the neurofeedback sessions were conducted did not seem to have a significant impact on the training's success in terms of alpha relative amplitude changes (0.04 ± 0.08 for silent room versus 0.07 ± 0.28 for noisy room, p = 0.740). However, the group exposed to intermittent noise appears to have favourable results in all performance assessments (p = 0.005 for working memory and p = 0.003 for reaction time). The results of the study suggested that performing neurofeedback training in an environment with intermittent noise can be interesting to athletes. Nevertheless, it is imperative to perform a replicated crossover design.

"REAC cervicobrachial neuromodulation treatment of depression, anxiety, and stress during the COVID-19 pandemic": Corrigendum

Reports an error in "REAC cervicobrachial neuromodulation treatment of depression, anxiety, and stress during the COVID-19 pandemic" by Ana Rita Pinheiro Barcessat, Marina Nolli Bittencourt, Larissa Duarte Ferreira, Erick de Souza Neri, Jose Alfredo Coelho Pereira, Fabio Bechelli and Arianna Rinaldi (Psychology Research and Behavior Management, 2020[Nov][9], Vol 13[929-937 ]). The authors have advised that there is an error in the author list on page 929. The author name "Erick de Souza Neri" should read "Erick Souza Neri". The name appears correctly in this and the original record. (The following abstract of the original article appeared in record 2021-08614-001). Introduction: In addition to the effects of coronavirus infection, the Covid-19 pandemic has induced widespread psychosocial distress, which has triggered the onset of anxious and depressive states, reactive to the socio-relational and economic situation induced by the pandemic. Some of our participants showed depressive and anxious attitudes even in the absence of real pictures of depression and anxiety. This phenomenon, combined with mechanisms of emulation and conditioning, can trigger a vicious cycle within interpersonal relationships and promote the administration of unnecessary treatments. Various approaches have been proposed to help populations suffering from psychosocial problems induced by the Covid-19 pandemic, but there is an objective difficulty in treating a large population. Methods: To contain and reduce this widespread psychosocial unease, in this study we used two radio electric asymmetric conveyer (REAC) technology neuromodulation treatments, neuro postural optimization (NPO) and neuropsychophysical optimization-cervicobrachial (NPPO-CB), aimed at optimizing an individual's response to the effects of environmental stressors. These treatments are quick and easy to administer;therefore, they can be administered to a large cohort of participants in a short time. To evaluate the effects of the REAC NPO and NPPO-CB treatments, the DASS-21 psychometric test was used because it has already been used to test depression, anxiety, and stress during the Covid-19 pandemic. Results: The results of the study confirm the usefulness of REAC NPO and NPPO-CB treatments in helping participants to have better coping strategies for the environmental pressures and reduce the neuropsychological and behavioral effects induced by the Covid-19 pandemic. Discussion: The results obtained in this study are consistent with previous clinical studies confirming the usefulness of the treatments to face neuropsychological and behavioral effects induced by exposome pressure. (PsycInfo Database Record (c) 2021 APA, all rights reserved)

Improving End-of-Life Care for People with Dementia in LTC Homes During the COVID-19 Pandemic and Beyond.

COVID-19 pandemic has resulted in a significant increase in deaths in long-term care homes (LTCH). People with dementia living in LTCHs represent one of the most frail and marginalized populations in Canada. The surge of COVID-19 cases in LTCHs and rationing of health-care resources during the pandemic have amplified the pre-existing need for improvements in palliative and end-of-life care in LTCHs. This position statement, created by a task force commissioned by the Alzheimer Society of Canada, provides recommendations for a multipronged coordinated approach to improving palliative and end-of-life care of people with dementia living in LTCHs during the COVID-19 pandemic and beyond.

Origin, phylogeny, variability and epitope conservation of SARS-CoV-2 worldwide.

The coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses innumerous challenges, like understanding what triggered the emergence of this new human virus, how this RNA virus is evolving or how the variability of viral genome may impact the primary structure of proteins that are targets for vaccine. We analyzed 19471 SARS-CoV-2 genomes available at the GISAID database from all over the world and 3335 genomes of other Coronoviridae family members available at GenBank, collecting SARS-CoV-2 high-quality genomes and distinct Coronoviridae family genomes. Additionally, we analyzed 199,984 spike glycoprotein sequences. Here, we identify a SARS-CoV-2 emerging cluster containing 13 closely related genomes isolated from bat and pangolin that showed evidence of recombination, which may have contributed to the emergence of SARS-CoV-2. The analyzed SARS-CoV-2 genomes presented 9632 single nucleotide variants (SNVs) corresponding to a variant density of 0.3 over the genome, and a clear geographic distribution. SNVs are unevenly distributed throughout the genome and hotspots for mutations were found for the spike gene and ORF 1ab. We describe a set of predicted spike protein epitopes whose variability is negligible. Additionally, all predicted epitopes for the structural E, M and N proteins are highly conserved. The amino acid changes present in the spike glycoprotein of variables of concern (VOCs) comprise between 3.4% and 20.7% of the predicted epitopes of this protein. These results favors the continuous efficacy of the available vaccines targeting the spike protein, and other structural proteins. Multiple epitopes vaccines should sustain vaccine efficacy since at least some of the epitopes present in variability regions of VOCs are conserved and thus recognizable by antibodies.

REAC Cervicobrachial Neuromodulation Treatment of Depression, Anxiety, and Stress During the COVID-19 Pandemic.

INTRODUCTION: In addition to the effects of coronavirus infection, the Covid-19 pandemic has induced widespread psychosocial distress, which has triggered the onset of anxious and depressive states, reactive to the socio-relational and economic situation induced by the pandemic. Some of our participants showed depressive and anxious attitudes even in the absence of real pictures of depression and anxiety. This phenomenon, combined with mechanisms of emulation and conditioning, can trigger a vicious cycle within interpersonal relationships and promote the administration of unnecessary treatments. Various approaches have been proposed to help populations suffering from psychosocial problems induced by the Covid-19 pandemic, but there is an objective difficulty in treating a large population. METHODS: To contain and reduce this widespread psychosocial unease, in this study we used two radio electric asymmetric conveyer (REAC) technology neuromodulation treatments, neuro postural optimization (NPO) and neuropsychophysical optimization-cervicobrachial (NPPO-CB), aimed at optimizing an individual's response to the effects of environmental stressors. These treatments are quick and easy to administer; therefore, they can be administered to a large cohort of participants in a short time. To evaluate the effects of the REAC NPO and NPPO-CB treatments, the DASS-21 psychometric test was used because it has already been used to test depression, anxiety, and stress during the Covid-19 pandemic. RESULTS: The results of the study confirm the usefulness of REAC NPO and NPPO-CB treatments in helping participants to have better coping strategies for the environmental pressures and reduce the neuropsychological and behavioral effects induced by the Covid-19 pandemic. DISCUSSION: The results obtained in this study are consistent with previous clinical studies confirming the usefulness of the treatments to face neuropsychological and behavioral effects induced by exposome pressure.

COVID-19 Personal Protective Equipment in the Home: Navigating the Complexity of Donning and Doffing.

PURPOSE: The safety of care professionals and patients is paramount while caring for people with infectious diseases, including those with confirmed or suspected severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and coronavirus disease 2019 (COVID-19). Existing policies and protocols for donning and doffing personal protective equipment (PPE) are primarily for institutional settings such as hospitals, not for home visits for patient care. We describe a protocol for donning and doffing PPE in home settings. METHODS: We used an iterative, rapid-prototyping approach to develop the protocol. A small workgroup created preliminary drafts, drawing on hospital-based protocols and modifying them, while undertaking simulations. Wider input was solicited via 2 webinars; 1 regional (Hamilton, Ontario) with palliative clinicians, and 1 national (Canada) with varying professions. We also consulted a group of infectious disease experts. A "how-to" video accompanies the protocol. RESULTS: Twelve versions of the protocol were produced, with major changes occurring within the first 6 versions. A national webinar mid-development provided further validation and minor modifications. Subsequent versions involved minor changes. The protocol has 4 phases: (1) Preparing, (2) Entering the Home, (3) Leaving the Home, and (4) After the Visit and Reprocessing. In addition to PPE-related equipment, the protocol requires additional materials including 2 pails for transporting supplies, plastic bags, hand sanitizer, disinfectant wipes, and printed easy-to-use checklists. CONCLUSIONS: This protocol addresses gaps in COVID-19-related guidelines, specifically the process of donning and doffing PPE during home visits while supplementing jurisdictional PPE guidelines and protocols.Appeared as Annals "Online First" article.

Shortages of Palliative Care Medications in Canada during the COVID-19 Pandemic: Gambling with Suffering.

Patients with serious illnesses such as cancer, advanced organ failure, dementia and COVID-19 rely on medications to alleviate suffering from uncontrolled symptoms. Numerous actual or threatened shortages of key medications used to provide palliation have been reported during the COVID-19 pandemic. This article explores the nature of these shortages, factors that have contributed to them and strategies to mitigate them. It calls on all levels of the healthcare system and the government to address this problem. Shortages in these medications are as serious as shortages in medications used to cure or control diseases.

Situación de la cirugía laparoscópica y robótica urológica durante la pandemia COVID-19

OBJETIVO: La pandemia provocada por el nuevo coronavirus SARS-CoV-2 ha tenido una elevada repercusión sobre la cirugía mínimamente invasiva (CMI). Ha surgido una importante controversia sobre la realización de CMI durante la pandemia COVID-19. Es prioritario, establecer un consenso sobre la organización y realización con seguridad de la CMI durante la pandemia. MATERIAL Y MÉTODOS: Se realizó una búsqueda web y en PubMed con los términos: "SARS-CoV-2", "COVID19", "COVID19 Urology", "COVID19 Surgery", "COVID19 transmission", "SARS-CoV-2 transmission", "COVID19 and minimally invasive surgery", "SARSCoV-2 and CO2 insuflation". Se realizó una revisión narrativa de la literatura y una síntesis de la evidencia disponible. Se ha utilizado una técnica de grupo nominal modificada, circulando un primer borrador a todos los autores y aprobándose la versión definitiva el día 26 de Mayo de 2020. RESULTADOS: No existe evidencia sobre una mayor exposición a SARS-CoV-2 en CMI respecto a cirugía abierta. La CMI se asocia a una menor estancia hospitalaria por lo que cambiar, sin justificación, la indicaciónde CMI puede retrotraer recursos que podrían ser utilizados para la pandemia COVID-19. Se debe priorizar la CMI según los recursos disponibles y la intensidad de la pandemia en cada momento. Se recomienda realizar despistaje de SARS-CoV-2 mediante cuestionario clínico-epidemiológico y PCR nasofaríngea 72 horas antes de la CMI electiva, para minimizar las complicaciones postoperatorias, evitar la transmisión cruzada entre pacientes y la posible exposición de los profesionales sanitarios. Se recomienda establecer medidas de organización en quirófano, de protección personal, técnica quirúrgica y manejo del CO2 y aerosoles generados para reducir la exposición y riesgos del personal sanitario. CONCLUSIONES: La CMI realizada con las medidasd e seguridad adecuadas para el paciente y profesionales, puede contribuir durante la desescalada a una menor utilización de recursos sanitarios y por tanto, no debe limitarse su utilización o cambiar sus indicaciones OBJECTIVE: SARS-CoV-2 pandemic has high repercussion on urologic minimally invasive surgery (MIS). Controversy about safety of MIS procedures during COVID-19 pandemic has been published. Nowadays, our priority should be create agreement in order to restart and organize MIS with safety conditions for patients and healthcare workers. METHODS: Pubmed and web search was conducted with following terms: "SARS-CoV-2", "COVID19";"COVID19 Urology", COVID19 Surgery", "COVID19 transmission", "SARS-CoV-2 transmission", "COVID19 and minimally invasive surgery""SARS-CoV-2 and CO2 insuflation". A narrative review of available literature and scientific evidence summary was done. A modify nominal group technique was used to achieve an expert consensus. First draft was circulated amongst authors. Definitive document was approved in May 26th. RESULTS: Non evidence supports higher risk of SARSCoV-2 healthcare workers infection with MIS compared to open surgery. MIS is associated with shorter hospital stay than open surgery. Modify MIS indications to open surgery, with no scientific evidence, could spend valuable resources in detriment to COVID-19 patients. MIS indications should be prioritized attending to available resources and pandemic intensity. SARS-CoV-2 screening 72 hours prior to surgery by clinical and epidemiological questionnaire and nasopharyngeal PCR is recommended, in order to prevent nosocomial transmission, professional infections and to minimize postoperative complications. Intraoperative steps should be established to reduce professional exposure to surgical aerosols, including: surgical room reorganization, adequate personal protective equipment, surgical technique optimization and management of CO2 and surgical smoke

Abdominal Wall Surgery after SARS-CoV-2: Time to Reestablish Postponed Non-essential Procedures?

Pandemic by the COVID-19 has found us unprotected to provide an adequate and rapid sanitary response. The hospital network of our public health system has provided most of the resources for the treatment of patients affected by the infection. Non-essential (non-priority) surgeries have been postponed. The optimal and proportionate reestablishment of these non-priority surgeries can be a problem. This article offers a technical and non-technical view of reestablishment non-priority surgeries from the perspective of abdominal wall surgery. Resumen La pandemia por el COVID-19 nos ha encontrado desprotegidos ante la dificultad para dar una respuesta sanitaria adecuada y rápida. La red de hospitales del sistema sanitario público ha dispuesto la mayoría de los recursos para el tratamiento de los pacientes afectos por la infección. Las cirugías no esenciales (no prioritarias) han sido aplazadas. El reinicio óptimo y proporcionado de estas cirugías no prioritarias puede representar un problema. En el presente artículo se ofrece una perspectiva técnica y no técnica del reinicio de las cirugías no prioritarias desde la óptica de la cirugía de la pared abdominal.

[Robotic and laparosocpic urological surgery during COVID-19 pandemia.]

OBJECTIVE: SARS-CoV-2 pandemic hashigh repercussion on urologic minimally invasive surgery (MIS). Controversy about safety of MIS procedures during COVID-19 pandemic has been published. Nowadays, our priority should be create agreement in order to restart and organize MIS with safety conditions for patients and healthcare workers. METHODS: Pubmed and web search was conducted with following terms: "SARS-CoV-2", "COVID19", "COVID19 Urology", COVID19 Surgery", "COVID19 transmission", "SARS-CoV-2 transmission", "COVID19 nd minimally invasive surgery", "SARS-CoV-2 and CO 2insuflation". A narrative review of available literature and scientific evidence summary was done. A modify nominal group technique was used to achieve an expert consensus. First draft was circulated amongst authors. Definitive document was approved in May 26th. RESULTS: Non evidence supports higher risk of SARSCoV-2 healthcare workers infection with MIS compared to open surgery. MIS is associated with shorter hospital stay than open surgery. Modify MIS indications to open surgery, with no scientific evidence, could spend valuable resources in detriment to COVID-19 patients. MIS indications should be prioritized attending to available resources and pandemic intensity. SARS-CoV-2screening 72 hours prior to surgery by clinical and epidemiological questionnaire and nasopharyngeal PCRis recommended, in order to prevent nosocomial transmission, professional infections and to minimize postoperative complications. Intraoperative steps should be established to reduce professional exposure to surgical aerosols, including: surgical room reorganization, adequate personal protective equipment, surgical technique optimization and management of CO2 and surgical smoke. CONCLUSIONS: In COVID-19 pandemic de-escalation, MIS carried out with optimal safety measurements, could contribute to reduce hospital resources utilization. With current evidence, MIS should not be limited or reconverted to open surgery during COVID-19 pandemic.

[Abdominal Wall Surgery After SARS-CoV-2: Time to Reestablish Postponed Non-essential Procedures?] / Cirugía de la pared abdominal después del SARS-CoV-2: ¿es hora de restablecer los procedimientos no esenciales pospuestos?

Pandemic by the COVID-19 has found us unprotected to provide an adequate and rapid sanitary response. The hospital network of our public health system has provided most of the resources for the treatment of patients affected by the infection. Non-essential (non-priority) surgeries have been postponed. The optimal and proportionate reestablishment of these non-priority surgeries can be a problem. This article offers a technical and non-technical view of reestablishment non-priority surgeries from the perspective of abdominal wall surgery.